Petrucco Stefania, Percudani Riccardo
Department of Biochemistry and Molecular Biology, University of Parma, Italy.
FEBS J. 2008 Mar;275(5):883-93. doi: 10.1111/j.1742-4658.2008.06259.x. Epub 2008 Jan 19.
PARP-like zinc fingers (zf-PARPs) are protein domains apt to the recognition of multiple DNA secondary structures. They were initially described as the DNA-binding, nick-sensor domains of poly(ADP-ribose)polymerases (PARPs). It now appears that zf-PARPs are evolutionary conserved in the eukaryotic lineage and associated with various enzymes implicated in nucleic acid transactions. In the present study, we discuss the functional and structural data of zf-PARPSs in the light of a comparative analysis of the protein family. Sequence and structural analyses allow the definition of the conserved features of the zf-PARP domain and the identification of five distinct phylogenetic groups. Differences among the groups accumulate on the putative DNA binding surface of the PARP zinc-finger fold. These observations suggest that different zf-PARP types have distinctive recognition properties for DNA secondary structures. A comparison of various functional studies confirms that the different finger types can accomplish a selective recognition of DNA structures.
聚（ADP - 核糖）聚合酶样锌指（zf - PARPs）是易于识别多种DNA二级结构的蛋白质结构域。它们最初被描述为聚（ADP - 核糖）聚合酶（PARPs）的DNA结合、切口传感器结构域。现在看来，zf - PARPs在真核生物谱系中是进化保守的，并且与参与核酸交易的各种酶相关。在本研究中，我们根据对该蛋白质家族的比较分析来讨论zf - PARPSs的功能和结构数据。序列和结构分析允许定义zf - PARP结构域的保守特征，并识别出五个不同的系统发育组。这些组之间的差异集中在PARP锌指折叠的假定DNA结合表面上。这些观察结果表明，不同类型的zf - PARP对DNA二级结构具有独特的识别特性。对各种功能研究的比较证实，不同的指型可以实现对DNA结构的选择性识别。
