Cheng Haiyan, Yu Jianchun, Jiang Zhiguo, Zhang Xuezhu, Liu Cunzhi, Peng Yingmei, Chen Fuyan, Qu You, Jia Yujie, Tian Qingfei, Xiao Chuan, Chu Qin, Nie Kun, Kan Bohong, Hu Xiaolin, Han Jingxian
Tianjin Key Laboratory of Acupuncture and Moxibustion, Molecular Biology Laboratory, Acupuncture and Moxibustion Research Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Neurosci Lett. 2008 Feb 20;432(2):111-6. doi: 10.1016/j.neulet.2007.12.009. Epub 2007 Dec 15.
Senescence-accelerated mouse prone 8 (SAMP8) is an autogenic senile strain characterized by early cognitive impairment and age-related deterioration of learning and memory. To investigate the effect of acupuncture on behavioral changes and brain cell events, male 4-month-old SAMP8 and age-matched homologous normal aging SAMR1 mice were divided into four groups: SAMP8 acupuncture group (Pa), SAMP8 non-acupoint control group (Pn), SAMP8 control group (Pc) and SAMR1 normal control group (Rc). By Morris water maze test, the cognitive deficit of SAMP8 was revealed and significantly improved by "Yiqitiaoxue and Fubenpeiyuan" acupuncture. Meanwhile, by 5'-bromo-2'-deoxyuridine (BrdU) specific immunodetection, the decreased cell proliferation in dentate gyrus (DG) of SAMP8 was greatly enhanced by the therapeutic acupuncture, suggesting acupoint-related specificity. Even though no significant differences were found in ventricular/subventricular zones (VZ/SVZ) of the third ventricle (V3) and lateral ventricle (LV) between groups, we obtained interesting results: a stream-like distribution of newly proliferated cells presented along the dorsum of alveus hippocampi (Alv), extending from LV to corpus callosum (CC), and the therapeutic acupuncture showed a marked effect on this region. Our research suggests that acupuncture can induce different cell proliferation in different brain regions of SAMP8, which brings forth the need to explore further for the mechanism of cognitive deficits and acupuncture intervention in this field.
衰老加速小鼠8型（SAMP8）是一种自发性衰老品系，其特征为早期认知障碍以及与年龄相关的学习和记忆衰退。为了研究针刺对行为变化和脑细胞活动的影响，将4月龄雄性SAMP8小鼠和年龄匹配的同源正常衰老SAMR1小鼠分为四组：SAMP8针刺组（Pa）、SAMP8非穴位对照组（Pn）、SAMP8对照组（Pc）和SAMR1正常对照组（Rc）。通过莫里斯水迷宫试验，揭示了SAMP8的认知缺陷，且“益气调血、扶本培元”针刺显著改善了该缺陷。同时，通过5'-溴-2'-脱氧尿苷（BrdU）特异性免疫检测发现，治疗性针刺极大地增强了SAMP8小鼠齿状回（DG）中降低的细胞增殖，提示穴位相关特异性。尽管各组间第三脑室（V3）和侧脑室（LV）的脑室下区/室管膜下区（VZ/SVZ）未发现显著差异，但我们获得了有趣的结果：新增殖细胞呈溪流状分布于海马槽（Alv）背侧，从LV延伸至胼胝体（CC），且治疗性针刺对该区域有显著影响。我们的研究表明，针刺可诱导SAMP8小鼠不同脑区出现不同的细胞增殖，这为进一步探索该领域认知缺陷及针刺干预机制提出了需求。
