Acupuncture and Moxibustion College, Chengdu University of Traditional Chinese Medcine, Chengdu, Sichuan, 610075, China.
Exp Gerontol. 2011 Oct;46(10):787-93. doi: 10.1016/j.exger.2011.06.002. Epub 2011 Jun 29.
A (1)H NMR-based metabonomic method was used to investigate the metabolic change of plasma in senescence-prone 8 (SAMP8) mice before and after electro-acupuncture (EA). Sixteen SAMP8 male mice (aged 8 months) were randomly divided into model group and acupuncture treatment group while the later group received EA treatment for 21 days. Eight senescence-resistant 1 (SAMR1) mice were used as the control group. Morris water maze was used to evaluate the effects of EA. All mice plasma samples obtained from different groups were analyzed by using 600 MHz (1)H nuclear magnetic resonances ((1)H NMR) spectroscopy. The data sets were analyzed by Principal Components Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) to discriminate the key plasma metabolites among different groups. Results indicated that both the escape and probe tasks of SAMP8 could be improved by EA treatment. Metabonomic study showed that SAMR1 and SAMP8 were separated clearly in both CPMG_OSC_PLS and LED _OSC_PLS score plots. Interestingly, samples obtained from EA group were distributed closely to SAMR1 group in CPMG_OSC_PLS score plot, but away from SAMP8 group in LED_OSC_PLS score plot. Corresponding loading plots showed that much less lactate was seen in SAMP8 mice plasma. Other changes including higher levels of dimethylamine (DMA) Choline and α-glucose but lower levels of leucine/isoleucine, HDL, LDL/VLDL, 3-Hydroxybutyrate (3-HB), and Trimethylamine N-oxide (TMAO) were observed in the SAMP8 mice plasma than in the SAMR1. After EA treatment, the levels of lactate, DMA, choline and TMAO were improved. Results of this work can provide valuable clues to the understanding of the metabolic changes in the senile impairment of mice. It is also hoped that the methodology can be used in evaluating the effects of EA and understanding the underlying acupuncture mechanism in treating neurodegenerative diseases.
一种基于（1）H NMR 的代谢组学方法被用于研究电针对衰老易感性 8 号（SAMP8）小鼠血浆代谢的影响。16 只雄性 SAMP8 小鼠（8 月龄）被随机分为模型组和针刺治疗组，后者接受 21 天电针治疗。8 只衰老抗性 1 号（SAMR1）小鼠被用作对照组。采用 Morris 水迷宫评价电针的效果。从不同组获得的所有小鼠血浆样本均采用 600MHz（1）H 核磁共振（（1）H NMR）光谱法进行分析。通过主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）对数据集进行分析，以区分不同组之间的关键血浆代谢物。结果表明，电针治疗可改善 SAMP8 的逃逸和探测任务。代谢组学研究表明，SAMR1 和 SAMP8 在 CPMG_OSC_PLS 和 LED _OSC_PLS 得分图中均清晰分离。有趣的是，CPMG_OSC_PLS 得分图中，电针组的样本分布与 SAMR1 组紧密相关，但在 LED_OSC_PLS 得分图中远离 SAMP8 组。相应的加载图显示，SAMP8 小鼠血浆中的乳酸含量较低。其他变化包括二甲基胺（DMA）胆碱和α-葡萄糖水平升高，但亮氨酸/异亮氨酸、高密度脂蛋白（HDL）、低密度脂蛋白/极低密度脂蛋白（LDL/VLDL）、3-羟丁酸（3-HB）和三甲胺 N-氧化物（TMAO）水平降低。电针治疗后，乳酸、DMA、胆碱和 TMAO 水平得到改善。这项工作的结果可以为理解小鼠衰老损伤的代谢变化提供有价值的线索。也希望该方法可用于评价电针的效果，了解治疗神经退行性疾病的针刺机制。
