高效液相色谱-二极管阵列检测法测定小鼠血浆中的亚胺培南和利福平。
Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection.
作者信息
Fernández-Torres R, Bello-López M A, Callejón-Mochón M, Jiménez-Sánchez J C
机构信息
Department of Analytical Chemistry, Faculty of Chemistry, 41012 Seville, Spain.
出版信息
Anal Chim Acta. 2008 Feb 11;608(2):204-10. doi: 10.1016/j.aca.2007.12.026. Epub 2008 Jan 3.
A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively. A linear response was observed at plasma levels ranged between 0.3 and 30 microgmL(-1) for imipenem and 1.5 and 20 microgmL(-1) for rifampicin. The detection limits were 0.07 microgmL(-1) and 0.47 microgmL(-1) for imipenem and rifampicin, respectively. The method was applied to the determination of both compounds in mouse plasma samples.
建立并验证了一种用于测定亚胺培南和利福平的高效液相色谱（HPLC）方法。该方法包括用甲醇进行血浆脱蛋白、在RP - 18柱上进行梯度洗脱以及二极管阵列检测。使用由甲醇和0.2M硼酸盐缓冲液（pH 7.2）组成的流动相，在8分钟内完成分离。亚胺培南和利福平分别在300 nm和255 nm处检测。在亚胺培南血浆浓度范围为0.3至30 μg/mL（-1）以及利福平血浆浓度范围为1.5至20 μg/mL（-1）时观察到线性响应。亚胺培南和利福平的检测限分别为0.07 μg/mL（-1）和0.47 μg/mL（-1）。该方法应用于小鼠血浆样品中两种化合物的测定。
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