采用高效液相色谱-紫外检测法同时测定血浆中三种碳青霉烯类抗生素。
Simultaneous determination of three carbapenem antibiotics in plasma by HPLC with ultraviolet detection.
作者信息
Legrand Tiphaine, Chhun Stéphanie, Rey Elisabeth, Blanchet Benoît, Zahar Jean-Ralph, Lanternier Fanny, Pons Gérard, Jullien Vincent
机构信息
Université Paris Descartes, Inserm, U663, Service de Pharmacologie Clinique, Groupe Hospitalier Cochin-Saint-Vincent de Paul, AP-HP, 75014 Paris, France.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 15;875(2):551-6. doi: 10.1016/j.jchromb.2008.09.020. Epub 2008 Sep 25.
A simple, precise and accurate high-performance liquid chromatography (HPLC) method using ultraviolet (UV) detection has been developed for simultaneous determination of carbapenem antibiotics: imipenem, meropenem and ertapenem in human plasma. Samples were spiked with ceftazidime as internal standard and proteins were precipitated by acetonitrile. Separation was achieved on a C8 column with a mobile phase composed of phosphate buffer 0.1M (pH 6.8) and methanol in gradient elution mode. Detection was performed at 298 nm. Calibration curves were linear from 0.5 to 80 mg/L for each compound, with correlation coefficients over 0.997. Intra- and inter-day validation studies showed accuracy between -4.5 and 8.1% and precision below 10.4%. Mean recoveries were 82.2, 90.8 and 87.7% for imipenem, meropenem and ertapenem, respectively. This method provides a useful tool for the therapeutic drug monitoring of carbapenems.
已开发出一种简单、精确且准确的高效液相色谱（HPLC）方法，该方法采用紫外（UV）检测，用于同时测定人血浆中的碳青霉烯类抗生素：亚胺培南、美罗培南和厄他培南。样品中加入头孢他啶作为内标，并用乙腈沉淀蛋白质。在C8柱上进行分离，流动相由0.1M磷酸盐缓冲液（pH 6.8）和甲醇组成，采用梯度洗脱模式。检测在298nm波长下进行。每种化合物的校准曲线在0.5至80mg/L范围内呈线性，相关系数超过0.997。日内和日间验证研究表明，准确度在-4.5%至8.1%之间，精密度低于10.4%。亚胺培南、美罗培南和厄他培南的平均回收率分别为82.2%、90.8%和87.7%。该方法为碳青霉烯类药物的治疗药物监测提供了一种有用的工具。
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