Knaś M, Choromańska M, Karaszewska K, Dudzik D, Waszkiel D, Borzym-Kluczyk M, Zaniewska A, Zwierz K
Department of Pharmaceutical Biochemistry, Medical University of Białystok, ul. Mickiewicza 2, 15-230 Białystok, Poland.
Adv Med Sci. 2007;52:186-90.
The aim of this work was to evaluate the influence of HIV infection on the catabolism of glycoconjugates in the oral cavity, by determination of the activity of lysosomal exoglycosidases in mixed saliva.
The specific activities of the following exoglycosidases were tested: N-acetyl-beta-hexosaminidase (HEX), its isoenzymes A (HEX-A) and B (HEX-B), alpha-mannosidase (MAN), beta-galactosidase (GAL) and alpha-fucosidase (FUC).
A significant increase of activity of HEX-A, GAL and FUC, and a significant decrease of the activity of HEX-B was found, but no significant changes in the HEX and MAN activity we noted.
Our results indicate that following HIV infection, there is probably an increased rate of catabolism of glycoconjugates in saliva resulting from changes in the proportions of the activity of isoenzymes A and B of N-acetyl-beta-hexosaminidase, beta-galactosidase and alpha-fucosidase. An increase of HEXA activity can implicate the beginning of neoplastic changes developing in the oral cavity.
本研究旨在通过测定混合唾液中溶酶体外切糖苷酶的活性，评估HIV感染对口腔中糖缀合物分解代谢的影响。
检测了以下外切糖苷酶的比活性：N-乙酰-β-己糖胺酶（HEX）及其同工酶A（HEX-A）和B（HEX-B）、α-甘露糖苷酶（MAN）、β-半乳糖苷酶（GAL）和α-岩藻糖苷酶（FUC）。
发现HEX-A、GAL和FUC的活性显著增加，HEX-B的活性显著降低，但未观察到HEX和MAN活性有显著变化。
我们的结果表明，HIV感染后，由于N-乙酰-β-己糖胺酶、β-半乳糖苷酶和α-岩藻糖苷酶同工酶A和B活性比例的变化，唾液中糖缀合物的分解代谢速率可能增加。HEXA活性的增加可能意味着口腔中肿瘤性变化的开始。
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