Szajda Slawomir Dariusz, Snarska Jadwiga, Puchalski Zbigniew, Zwierz Krzysztof
Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Poland.
Hepatogastroenterology. 2008 May-Jun;55(84):921-5.
BACKGROUND/AIMS: The aim of this study is to evaluate the usefulness of determination the activity of lysosomal exoglycosidases: N-acetyl-beta-D-hexosaminidase (HEX - E.C. 3.2.1.30), beta-D-galactosidase (GAL - E.C. 3.2.1.23), alpha-L-fucosidase (FUC - E.C. 3.2.1.51) and alpha-D-mannosidase (MAN - E.C. 3.2.1.24) in blood serum and urine in diagnostics of colon adenocarcinoma.
The activity of lysosomal exoglycosidases was determined by the method of Marciniak et al. adapted to serum and urine of patients with adenocarcinoma of the colon.
A significant increase in concentration of the activity of HEX, GAL and FUC was found in blood serum, as well as HEX and GAL in urine, of patients with colon adenocarcinoma, in comparison with healthy people. With the method of Marciniak et al. for determination the activity of HEX, GAL and FUC in blood serum as well as HEX and GAL in urine, the cases of colon adenocarcinoma were significantly differentiated from healthy people.
The high diagnostic value, sensitivity and specificity of the method of Marciniak et al., suggests the possibility of its use for determination of the activity of HEX, FUC and GAL in blood serum as well as HEX and GAL in urine, in the diagnostics of colon adenocarcinoma.
背景/目的：本研究旨在评估测定血清和尿液中溶酶体外切糖苷酶的活性：N-乙酰-β-D-己糖胺酶（HEX - E.C. 3.2.1.30）、β-D-半乳糖苷酶（GAL - E.C. 3.2.1.23）、α-L-岩藻糖苷酶（FUC - E.C. 3.2.1.51）和α-D-甘露糖苷酶（MAN - E.C. 3.2.1.24）在结肠腺癌诊断中的作用。
采用Marciniak等人的方法测定溶酶体外切糖苷酶的活性，并将其应用于结肠腺癌患者的血清和尿液。
与健康人相比，结肠腺癌患者血清中HEX、GAL和FUC的活性浓度显著升高，尿液中HEX和GAL的活性浓度也显著升高。采用Marciniak等人的方法测定血清中HEX、GAL和FUC以及尿液中HEX和GAL的活性，结肠腺癌病例与健康人有显著差异。
Marciniak等人的方法具有较高的诊断价值、敏感性和特异性，提示其有可能用于测定血清中HEX、FUC和GAL以及尿液中HEX和GAL的活性，以诊断结肠腺癌。
