1型糖尿病患者的唾液：吸烟对溶酶体外切糖苷酶活性的影响。
Saliva of patients with Type 1 diabetes: effect of smoking on activity of lysosomal exoglycosidases.
作者信息
Knas M, Karaszewska K, Szajda S D, Zarzycki W, Dudzik D, Zwierz K
机构信息
Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland.
出版信息
Oral Dis. 2006 May;12(3):278-82. doi: 10.1111/j.1601-0825.2005.01190.x.
OBJECTIVE
The aim of our study was to evaluate the influence of smoking on the activity of N-acetyl-beta-hexosaminidase (HEX), its isoenzymes A (HEX-A) and B (HEX-B) and beta-galactosidase (GAL), in the saliva of patients with Type 1 diabetes.
METHODS
In the supernatant HEX and its isoenzymes A and B, and beta-galactosidase were determined by the method of Chatteriee et al in modification of Zwierz et al (mKat kg(-1) of protein). Protein was determined by the Lowry et al method (mg ml(-1)).
RESULTS
The results presented here suggest that diabetes and smoking modify activity of HEX and its isoenzymes, but only combination of diabetes and smoking give a significant increase in the specific activity of HEX and its isoenzymes.
CONCLUSIONS
Type 1 diabetes slightly changes the composition of saliva. Smoking cigarettes significantly modifies the composition and properties of saliva in healthy individuals and patients with Type 1 diabetes.
目的
我们研究的目的是评估吸烟对1型糖尿病患者唾液中N - 乙酰 - β - 己糖胺酶（HEX）及其同工酶A（HEX - A）和B（HEX - B）以及β - 半乳糖苷酶（GAL）活性的影响。
方法
采用Chatteriee等人改进的Zwierz等人的方法（每千克蛋白质的毫卡他单位）测定上清液中的HEX及其同工酶A和B以及β - 半乳糖苷酶。蛋白质采用Lowry等人的方法测定（毫克每毫升）。
结果
此处呈现的结果表明，糖尿病和吸烟会改变HEX及其同工酶的活性，但只有糖尿病和吸烟同时存在时，HEX及其同工酶的比活性才会显著增加。
结论
1型糖尿病会轻微改变唾液的成分。吸烟会显著改变健康个体和1型糖尿病患者唾液的成分和性质。
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