利用金纳米颗粒和核酸侧向流动（NALF）装置对PCR产物进行一步可视化检测。
One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device.
作者信息
Aveyard Jenny, Mehrabi Maryam, Cossins Andrew, Braven Helen, Wilson Robert
机构信息
Department of Chemistry, Liverpool University, Liverpool, L69 7ZD, UK.
出版信息
Chem Commun (Camb). 2007 Nov 7(41):4251-3. doi: 10.1039/b708859k.
DOI:10.1039/b708859k
PMID:18217596
Abstract
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
摘要
通过将特异性PCR产物夹在共价连接到金纳米颗粒（GNPs）上的报告寡核苷酸和共价连接到硝酸纤维素色谱条上的捕获寡核苷酸之间，使用无抗体侧向流动装置检测特异性PCR产物。
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