Biosensors and Nanotechnology Applications Laboratory, University of Idaho, Coeur d'Alene, ID 83814-2277, USA.
Chem Commun (Camb). 2012 Aug 11;48(62):7714-6. doi: 10.1039/c2cc33430e. Epub 2012 Jun 29.
A lateral flow test strip assay, enabling sensitive detection of DNA specific to the foodborne pathogen E. coli O157:H7, is described. The use of LNA-conjugated gold nanoparticle probes, along with signal amplification protocols, results in minimum detectable concentrations of ~0.4 nM.
本文描述了一种侧向流动测试条检测法,能够灵敏地检测食源性病原体大肠杆菌 O157:H7 的特异性 DNA。该方法使用了连接锁核酸(LNA)修饰的金纳米颗粒探针,并结合信号放大方案,将最低可检测浓度降低至约 0.4 nM。