State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing 210096, China.
Theranostics. 2019 Jul 9;9(17):4849-4859. doi: 10.7150/thno.35824. eCollection 2019.
Respiratory tract infections (RTIs) are severe acute infectious diseases, which require the timely and accurate identification of the pathogens involved so that the individual treatment plan can be selected, including optimized use of antibiotics. However, high throughput and ultrasensitive quantification of multiple nucleic acids is a challenge in a point of care testing (POCT) device. Herein, we developed a 2×3 microarray on a lateral flow strip with surface enhanced Raman scattering (SERS) nanotags encoding the nucleic acids of 11 common RTI pathogens. On account of the signal magnification of encoded SERS nanotags in addition to the high surface area to volume ratio of the nitrocellulose (NC) membrane, rapid quantification of the 11 pathogens with a broad linear dynamic range (LDR) and ultra-high sensitivity was achieved on one lateral flow microarray. The limit of detection (LOD) for influenza A, parainfluenza 1, parainfluenza 3, respiratory syncytial virus, coxiella burnetii, legionella pneumophila, influenza B, parainfluenza 2, adenovirus, chlamydophila pneumoniae, and mycoplasma pneumoniae were calculated to be 0.031 pM, 0.030 pM, 0.038 pM, 0.038 pM, 0.040 pM, 0.039 pM, 0.035 pM, 0.032 pM, 0.040 pM, 0.039 pM, and 0.041 pM, respectively. The LDR of measurement of the target nucleic acids of the eleven RTI pathogens were 1 pM-50 nM, which span 5 orders of magnitude. We anticipate this novel approach could be widely adopted in the early and precise diagnosis of RTI and other diseases.
呼吸道感染（RTIs）是严重的急性传染病，需要及时准确地识别病原体，以便选择个体化的治疗方案，包括优化抗生素的使用。然而，在即时检测（POCT）设备中，高通量和超灵敏地定量检测多种核酸是一个挑战。
在这里，我们在侧流条上开发了一个 2×3 的微阵列，该微阵列带有表面增强拉曼散射（SERS）纳米标签，可对 11 种常见 RTIs 病原体的核酸进行编码。由于编码的 SERS 纳米标签的信号放大以及硝酸纤维素（NC）膜的高表面积与体积比，我们可以在一个侧流微阵列上实现对 11 种病原体的快速定量检测，具有宽线性动态范围（LDR）和超高灵敏度。
流感 A、副流感 1、副流感 3、呼吸道合胞病毒、柯克斯体、嗜肺军团菌、流感 B、副流感 2、腺病毒、肺炎衣原体和肺炎支原体的检测限（LOD）分别计算为 0.031 pM、0.030 pM、0.038 pM、0.038 pM、0.040 pM、0.039 pM、0.035 pM、0.032 pM、0.040 pM、0.039 pM 和 0.041 pM。这 11 种 RTI 病原体的目标核酸的测量 LDR 为 1 pM-50 nM，跨越了 5 个数量级。
我们预计这种新方法可以广泛应用于 RTI 和其他疾病的早期和精确诊断。
