大麦凝集素羧基末端前肽是植物中的一种液泡蛋白分选决定因子。
The barley lectin carboxyl-terminal propeptide is a vacuolar protein sorting determinant in plants.
作者信息
Bednarek S Y, Raikhel N V
机构信息
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824-1312.
出版信息
Plant Cell. 1991 Nov;3(11):1195-206. doi: 10.1105/tpc.3.11.1195.
We have previously shown that the 15-amino acid carboxyl-terminal propeptide of probarley lectin is necessary for the proper sorting of this protein to the plant vacuole. A mutant form of the protein lacking the carboxyl-terminal propeptide is secreted. To test whether the carboxyl-terminal propeptide is the vacuole sorting determinant of probarley lectin, we examined in transgenic tobacco the processing and sorting of a series of fusion proteins containing the secreted protein, cucumber chitinase, and regions of probarley lectin. Pulse-labeling experiments demonstrated that the fusion proteins were properly translocated through the tobacco secretory system and that cucumber chitinase and cucumber chitinase fusion proteins lacking the carboxyl-terminal propeptide were secreted. The cucumber chitinase fusion protein containing the carboxyl-terminal propeptide was properly processed and sorted to the vacuole in transgenic tobacco as confirmed by organelle fractionation and electron microscopy immunocytochemistry. Therefore, the barley lectin carboxyl-terminal propeptide is both necessary and sufficient for protein sorting to the plant vacuole.
我们之前已经表明，大麦凝集素的15个氨基酸的羧基末端前肽对于该蛋白质正确分选至植物液泡是必需的。缺乏羧基末端前肽的该蛋白质的突变形式会被分泌。为了测试羧基末端前肽是否是大麦凝集素的液泡分选决定因素，我们在转基因烟草中检测了一系列融合蛋白的加工和分选情况，这些融合蛋白包含分泌蛋白、黄瓜几丁质酶以及大麦凝集素的区域。脉冲标记实验表明，融合蛋白能够正确地通过烟草分泌系统转运，并且缺乏羧基末端前肽的黄瓜几丁质酶和黄瓜几丁质酶融合蛋白会被分泌。通过细胞器分级分离和电子显微镜免疫细胞化学证实，含有羧基末端前肽的黄瓜几丁质酶融合蛋白在转基因烟草中能够被正确加工并分选至液泡。因此，大麦凝集素羧基末端前肽对于蛋白质分选至植物液泡既是必需的也是充分的。
Zotero 插件