O'Hern P A, Goldberg E
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.
Protein Expr Purif. 1991 Feb;2(1):59-62. doi: 10.1016/1046-5928(91)90010-g.
Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis.
将烷基碳官能团与琼脂糖珠偶联的开放式疏水相互作用色谱已用于对大量硫酸铵分级分离的细菌细胞裂解物进行脱盐。目标蛋白质可在不到4小时的时间内同时脱盐和浓缩，且产量明显优于通过沉淀、离心和透析的标准技术获得的产量。
