一种针对人癌胚抗原和铟-苄基-乙二胺四乙酸的嵌合双功能抗体的纯化与表征
Purification and characterization of a chimeric bifunctional antibody specific for human carcinoembryonic antigen and indium-benzyl-EDTA.
作者信息
Beidler D E, Johnson M J, Unger B W, Phelps J L, Jue R A
机构信息
Department of Protein Engineering, Hybritech Incorporated, San Diego, California 92196.
出版信息
Protein Expr Purif. 1991 Feb;2(1):75-82. doi: 10.1016/1046-5928(91)90014-a.
We describe the purification and characterization of a genetically engineered mouse/human chimeric bifunctional antibody specific for human carcinoembryonic antigen and indium-benzyl-EDTA. A clone expressing the bifunctional antibody has been previously described by our group and was found in this investigation also to express monospecific antibodies as well as Ig forms with mismatched light and heavy chains. The physicochemical properties of these various chimeric immunoglobulins were nearly identical. Isoelectric focusing showed that all these immunoglobulins have pI values between 8.47 and 8.80. A purification method that separates the bifunctional antibody from other Ig forms expressed in the same clone has been devised by relying on a unique interaction between the metal chelate binding region of these antibodies and the sulfopropyl functional group of a TSK SP 5-PW column.
我们描述了一种针对人癌胚抗原和铟-苄基-乙二胺四乙酸的基因工程小鼠/人嵌合双功能抗体的纯化及特性分析。我们小组之前已描述过一个表达双功能抗体的克隆，在本研究中还发现其表达单特异性抗体以及轻链和重链错配的Ig形式。这些各种嵌合免疫球蛋白的物理化学性质几乎相同。等电聚焦显示所有这些免疫球蛋白的pI值在8.47至8.80之间。通过利用这些抗体的金属螯合物结合区域与TSK SP 5-PW柱的磺丙基官能团之间的独特相互作用，设计出了一种从同一克隆中表达的其他Ig形式中分离双功能抗体的纯化方法。
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