Phelps J L, Beidler D E, Jue R A, Unger B W, Johnson M J
Department of Immunobiology, Hybritech, Inc., San Diego, CA 92121.
J Immunol. 1990 Aug 15;145(4):1200-4.
A simple method is described for the generation of a biologically produced mouse/human chimeric hetero-bifunctional antibody that has dual specificity for human carcinoembryonic Ag and metal chelate haptens. Two large compound chimeric vectors each containing the genetic information to produce a single antibody specificity were sequentially electroporated into the murine nonsecreting hybridoma SP2/0. This led to the isolation of a clone expressing high levels of total IgG (up to 25 micrograms/ml/10(6) cells), 10 to 20% of which showed simultaneous reactivity with both Ag. Binding studies showed that the immunoreactivities and affinity constants for the individual arms of the bifunctional antibody were equivalent to those seen with the parental antibodies.
描述了一种生成生物产生的小鼠/人嵌合异源双功能抗体的简单方法，该抗体对人癌胚抗原和金属螯合半抗原有双重特异性。将两个分别包含产生单一抗体特异性遗传信息的大型复合嵌合载体依次电穿孔导入小鼠非分泌性杂交瘤SP2/0。这导致分离出一个表达高水平总IgG（高达25微克/毫升/10⁶个细胞）的克隆，其中10%至20%的IgG显示出对两种抗原的同时反应性。结合研究表明，双功能抗体各臂的免疫反应性和亲和常数与亲本抗体相当。
