Yoon Jung-Hoon, Kang So-Jung, Lee Mi-Hwa, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):434-7. doi: 10.1099/ijs.0.65267-0.
A Gram-positive, non-motile, rod-shaped bacterial strain, DS-30(T), was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-30(T) grew optimally at pH 6.0-7.0 and 25 degrees C in the presence of 0-0.5 % (w/v) NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-30(T) fell within the genus Nocardioides, forming a coherent cluster with the type strains of Nocardioides aquiterrae and Nocardioides pyridinolyticus, with which it exhibited 16S rRNA gene sequence similarity values of 98.1 and 98.3 %, respectively. Strain DS-30(T) exhibited 16S rRNA gene sequence similarity values below 96.8 % with respect to the type strains of the other Nocardioides species. The chemotaxonomic properties of strain DS-30(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16 : 0), C(17 : 1)omega8c and C(18 : 1)omega9c. The DNA G+C content was 71.3 mol%. Strain DS-30(T) exhibited DNA-DNA relatedness levels of 19 and 22 % with respect to the type strains of N. aquiterrae and N. pyridinolyticus, respectively, and could be differentiated from these species with reference to a number of phenotypic characteristics. On the basis of the data obtained, strain DS-30(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides hankookensis sp. nov. is proposed. The type strain is DS-30(T) (=KCTC 19246(T) =CCUG 54522(T)).
从韩国独岛的土壤中分离出一株革兰氏阳性、无运动性、杆状的细菌菌株DS-30(T)，并通过多相分类研究对其分类地位进行了调查。菌株DS-30(T)在pH 6.0 - 7.0和25℃、0 - 0.5%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株DS-30(T)属于诺卡氏菌属，与水生诺卡氏菌和吡啶分解诺卡氏菌的模式菌株形成一个连贯的簇，与之16S rRNA基因序列相似性值分别为98.1%和98.3%。菌株DS-30(T)与其他诺卡氏菌属物种的模式菌株的16S rRNA基因序列相似性值低于96.8%。菌株DS-30(T)的化学分类学特性与诺卡氏菌属一致：细胞壁肽聚糖类型基于ll-二氨基庚二酸，主要甲基萘醌为MK-8(H(4))，主要脂肪酸为异C(16 : 0)、C(17 : 1)ω8c和C(18 : 1)ω9c。DNA G+C含量为71.3 mol%。菌株DS-30(T)与水生诺卡氏菌和吡啶分解诺卡氏菌的模式菌株的DNA-DNA相关性水平分别为19%和22%，并可根据一些表型特征与这些物种区分开来。根据获得的数据，菌株DS-30(T)代表诺卡氏菌属的一个新物种，为此提出名称韩国诺卡氏菌（Nocardioides hankookensis sp. nov.）。模式菌株为DS-30(T)（=KCTC 19246(T) =CCUG 54522(T)）。
