Yoon Jung-Hoon, Kang So-Jung, Park Sooyeon, Kim Wonyong, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology , Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2794-7. doi: 10.1099/ijs.0.010124-0. Epub 2009 Jul 23.
A Gram-positive, non-motile, rod- or coccoid-shaped bacterial strain, designated MN8(T), was isolated from sludge of domestic wastewater in Korea, and its taxonomic position was investigated by use of a polyphasic study. Strain MN8(T) grew optimally at pH 6.5-7.5 and 30 degrees C and in the presence of 0-0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MN8(T) fell within the cluster comprising Nocardioides species, clustering with Nocardioides simplex KCTC 9106(T), Nocardioides aromaticivorans H-1(T), Nocardioides kongjuensis A2-4(T) and Nocardioides nitrophenolicus NSP41(T), with which it shared 98.4-99.0 % 16S rRNA gene sequence similarity. The chemotaxonomic properties of strain MN8(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16 : 0) and C(18 : 1)omega9c. The DNA G+C content was 71.5 mol%. Strain MN8(T) exhibited levels of DNA-DNA relatedness of 13-31 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics. On the basis of the data presented, strain MN8(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides caeni sp. nov. is proposed. The type strain is MN8(T) (=KCTC 19600(T)=CCUG 57506(T)).
从韩国生活污水污泥中分离出一株革兰氏阳性、无运动性、杆状或球状的细菌菌株，命名为MN8(T)，并通过多相分类研究对其分类地位进行了调查。菌株MN8(T)在pH 6.5 - 7.5和30℃以及0 - 0.5%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株MN8(T)属于包含诺卡氏菌属物种的簇，与简单诺卡氏菌KCTC 9106(T)、芳香诺卡氏菌H-1(T)、公州诺卡氏菌A2-4(T)和硝基酚诺卡氏菌NSP41(T)聚类，与它们共享98.4 - 99.0%的16S rRNA基因序列相似性。菌株MN8(T)的化学分类学特性与诺卡氏菌属一致：细胞壁肽聚糖类型基于ll-2,6-二氨基庚二酸，主要甲基萘醌是MK-8(H(4))，主要脂肪酸是异-C(16 : 0)和C(18 : 1)ω9c。DNA G+C含量为71.5 mol%。菌株MN8(T)与系统发育相关的诺卡氏菌属模式菌株的DNA-DNA相关性水平为13 - 31%，并且可以根据表型特征的差异与这些物种区分开来。根据所提供的数据，菌株MN8(T)被认为代表诺卡氏菌属的一个新物种，为此提出新名称诺卡氏菌新种（Nocardioides caeni sp. nov.）。模式菌株是MN8(T)（=KCTC 19600(T)=CCUG 57506(T)）。
