Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Uttar Pradesh, India.
Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India.
Methods Mol Biol. 2020;2107:127-145. doi: 10.1007/978-1-0716-0235-5_6.
Methylation of cytosines in DNA is the most stable type of epigenetic modification that is established and maintained by different enzymes. In plants, DNA methylation is inherited from one generation to another leaving an epigenetic mark as a memory of previous state, which may include encounter with stress or pathogen. Advancement in the next generation sequencing technologies has enabled the profiling of methylation marks. Whole-genome bisulfite sequencing (WGBS) has the potential to unravel the patterns of DNA methylation at single-base resolution. Though the sequencing technologies have evolved drastically, analysis of WGBS data still remains challenging. Here, we provide a methodology for performing WGBS data analysis along with critical steps for identification of methylation marks in plant genomes including legumes.
DNA 中的胞嘧啶甲基化是最稳定的表观遗传修饰类型，由不同的酶建立和维持。在植物中，DNA 甲基化从一代遗传到另一代，留下表观遗传标记作为先前状态的记忆，其中可能包括遇到压力或病原体。下一代测序技术的进步使甲基化标记的分析成为可能。全基因组亚硫酸氢盐测序（WGBS）有可能以单碱基分辨率揭示 DNA 甲基化的模式。尽管测序技术有了很大的发展，但 WGBS 数据的分析仍然具有挑战性。在这里，我们提供了一种进行 WGBS 数据分析的方法，以及在包括豆科植物在内的植物基因组中识别甲基化标记的关键步骤。
