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本文引用的文献

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Highly integrated single-base resolution maps of the epigenome in Arabidopsis.拟南芥表观基因组的高度整合单碱基分辨率图谱
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Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning.拟南芥基因组的鸟枪法亚硫酸氢盐测序揭示了DNA甲基化模式。
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Epigenetic inheritance in plants.植物中的表观遗传继承
Nature. 2007 May 24;447(7143):418-24. doi: 10.1038/nature05917.
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Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription.拟南芥DNA甲基化的全基因组分析揭示了甲基化与转录之间的相互依存关系。
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Genome-wide high-resolution mapping and functional analysis of DNA methylation in arabidopsis.拟南芥中DNA甲基化的全基因组高分辨率图谱绘制与功能分析
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Hairpin-bisulfite PCR: assessing epigenetic methylation patterns on complementary strands of individual DNA molecules.发夹亚硫酸氢盐PCR：评估单个DNA分子互补链上的表观遗传甲基化模式。
Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):204-9. doi: 10.1073/pnas.2536758100. Epub 2003 Dec 12.
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High sensitivity mapping of methylated cytosines.甲基化胞嘧啶的高灵敏度图谱分析。
Nucleic Acids Res. 1994 Aug 11;22(15):2990-7. doi: 10.1093/nar/22.15.2990.
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Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.位点特异性修饰对限制性核酸内切酶和DNA修饰甲基转移酶的影响。
Nucleic Acids Res. 1994 Sep;22(17):3640-59. doi: 10.1093/nar/22.17.3640.
9
A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.一种基因组测序方案，可在单条DNA链中对5-甲基胞嘧啶残基进行阳性显示。
Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1827-31. doi: 10.1073/pnas.89.5.1827.

准确的植物亚硫酸氢盐测序。

Accurate sodium bisulfite sequencing in plants.

机构信息

Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, UK.

出版信息

Epigenetics. 2010 Jan 1;5(1):47-9. doi: 10.4161/epi.5.1.10560. Epub 2010 Jan 8.

DOI:10.4161/epi.5.1.10560
PMID:20081358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2829377/
Abstract

DNA cytosine methylation is a conserved epigenetic modification frequently correlating with transcriptional silencing in a wide variety of eukaryotic organisms. Sodium bisulfite treatment of DNA converts unmethylated cytosine to uracil, while 5-methylated cytosine is protected. We describe techniques that ensure reliable sequencing data following sodium bisulfite conversion and to avoid common pitfalls such as amplification of unconverted DNA and inclusion of sibling clones.

摘要

DNA 胞嘧啶甲基化是一种保守的表观遗传修饰，常与真核生物中广泛的转录沉默相关。DNA 的亚硫酸氢钠处理将未甲基化的胞嘧啶转化为尿嘧啶，而 5-甲基化的胞嘧啶则受到保护。我们描述了确保亚硫酸氢钠转化后获得可靠测序数据的技术，并避免了常见的陷阱，如未转化 DNA 的扩增和同系克隆的包含。