Martins C M O, Fernandes B F, Antecka E, Di Cesare S, Mansure J J C, Marshall J-C, Burnier M N
Department of Ophthalmology, McGill University Health Centre, Montreal, Quebec, Canada.
Eye (Lond). 2008 May;22(5):707-11. doi: 10.1038/sj.eye.6703090. Epub 2008 Jan 25.
Uveal melanoma (UM) is the most common primary malignant intraocular tumour in adults. Forty-five percent of UM patients develop metastasis within 15 years of initial diagnosis. KISS1, a human metastasis suppressor gene, has been reported to play a role in various human malignancies. The purpose of this study was to investigate the expression of KISS1 in UM and its potential value as a prognostic marker.
Thirty-seven cases of paraffin-embedded human UM specimens were immunostained with a KISS1 antibody. Clinical-pathological data were obtained. The relationship between the clinical-pathological data and the expression of KISS1 was evaluated. Moreover, the survival rates of the patients were also assessed. Five UM cell lines (92.1, OCM-1, MKTBR, UW1 and SP6.5) were assayed for KISS1 expression. In addition, real-time PCR was used to determine mRNA levels of KISS1and its receptor GPR54in these cell lines.
The immunohistochemical results of KISS1 expression displayed cytoplasmic staining in 84% of UM specimens. Low KISS1 expression was associated with a higher risk of metastatic disease (P<0.05). Furthermore, we found that KISS1 was expressed in all five UM cells lines. Real-time PCR analysis confirmed the presence of both KISS1and its receptor GPR54in all five human UM cell lines.
To the best of our knowledge, this is the first time that KISS1has been characterized in UM. The correlation between KISS1 expression and UM survival rate suggests an important role for KISS1as a prognostic marker in this particular tumour.
葡萄膜黑色素瘤（UM）是成人中最常见的原发性眼内恶性肿瘤。45%的UM患者在初次诊断后的15年内发生转移。KISS1是一种人类转移抑制基因，据报道在多种人类恶性肿瘤中发挥作用。本研究的目的是调查KISS1在UM中的表达及其作为预后标志物的潜在价值。
用KISS1抗体对37例石蜡包埋的人类UM标本进行免疫染色。获取临床病理数据。评估临床病理数据与KISS1表达之间的关系。此外，还评估了患者的生存率。检测了5种UM细胞系（92.1、OCM-1、MKTBR、UW1和SP6.5）的KISS1表达。此外，使用实时PCR测定这些细胞系中KISS1及其受体GPR54的mRNA水平。
KISS1表达的免疫组化结果显示，84%的UM标本中有细胞质染色。低KISS1表达与转移疾病的高风险相关（P<0.05）。此外，我们发现KISS1在所有5种UM细胞系中均有表达。实时PCR分析证实，在所有5种人类UM细胞系中均存在KISS1及其受体GPR54。
据我们所知，这是首次在UM中对KISS1进行表征。KISS1表达与UM生存率之间的相关性表明KISS1作为这种特定肿瘤的预后标志物具有重要作用。
