Genetics and Molecular Biology Research Unit (UPGEM), Faculdade de Medicina de São José do Rio Preto-FAMERP, São José do Rio Preto, SP, CEP 15090-000, Brazil.
Mol Biol Rep. 2012 May;39(5):6029-34. doi: 10.1007/s11033-011-1416-8. Epub 2012 Jan 1.
The KiSS-1 metastasis-suppressor gene (KiSS-1) product (metastin, kisspeptin) is reported to act after binding with the natural ligand of a G-protein coupled receptor and this gene product inhibits chemotaxis, invasion, and metastasis of cells. The aim of this study was to evaluate the Q36R polymorphism of KiSS-1 in patients with head and neck cancer and to compare the results with healthy individuals and its association with clinicopathological parameters. Gender, age, smoking and alcohol consumption were analyzed for 744 individual (252 head and neck cancer patients and in 522 control individuals). The molecular analysis of these individuals was made after extraction of genomic DNA using the SSCP-PCR technique. This study did not reveal any significant differences in genotype frequencies between healthy individuals and patients with head and neck cancer or with the clinical parameters. This study showed an increase frequency of the Q36R polymorphism in pharyngeal cancer.
KiSS-1 转移抑制基因（KiSS-1）产物（促黑色素细胞激素，kisspeptin）据报道在与 G 蛋白偶联受体的天然配体结合后发挥作用，该基因产物抑制细胞的趋化性、侵袭和转移。本研究旨在评估头颈部癌症患者中 KiSS-1 的 Q36R 多态性，并将结果与健康个体进行比较及其与临床病理参数的关系。分析了 744 名个体（252 名头颈部癌症患者和 522 名对照个体）的性别、年龄、吸烟和饮酒情况。使用 SSCP-PCR 技术提取基因组 DNA 后，对这些个体进行了分子分析。本研究未发现健康个体与头颈部癌症患者或与临床参数之间基因型频率有任何显著差异。本研究显示咽癌中 Q36R 多态性的频率增加。
