SPARC在人葡萄膜黑色素瘤中表达，其缺失会降低肿瘤细胞的增殖。
SPARC is expressed in human uveal melanoma and its abrogation reduces tumor cell proliferation.
作者信息
Maloney Shawn C, Marshall Jean-Claude, Antecka Emilia, Orellana Maria Eugenia, Fernandes Bruno F, Martins Claudia, Castiglione Enzo, DI Cesare Sebastian, Logan Patrick, Burnier Miguel N
机构信息
The Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, Canada.
出版信息
Anticancer Res. 2009 Aug;29(8):3059-64.
BACKGROUND
Secreted protein acidic and rich in cysteine (SPARC) has been shown to play an integral role in the progression of numerous malignancies. The aim of this study was to investigate the expression of SPARC in uveal melanoma (UM).
MATERIALS AND METHODS
SPARC expression was assessed in UM cell lines using RT-PCR and immunocytochemistry. Small interfering RNA directed against SPARC was used to transfect each of the cell lines, which were subsequently run in proliferation assays. SPARC expression was further investigated in 19 cases of human UM and 11 primary and 8 metastatic tumors from a rabbit xenograft model.
RESULTS
The cell lines transfected with SPARC siRNA showed a significant decrease in proliferation compared to controls. All cases of human uveal melanoma demonstrated positive staining for SPARC as did all primary and metastatic tumors from the xenograft model.
CONCLUSION
SPARC may represent a novel target to inhibit growth of UM.
背景
富含半胱氨酸的酸性分泌蛋白（SPARC）已被证明在多种恶性肿瘤的进展中发挥着不可或缺的作用。本研究的目的是调查SPARC在葡萄膜黑色素瘤（UM）中的表达情况。
材料与方法
使用逆转录聚合酶链反应（RT-PCR）和免疫细胞化学方法评估UM细胞系中SPARC的表达。针对SPARC的小干扰RNA用于转染每个细胞系，随后进行增殖试验。在19例人类UM以及来自兔异种移植模型的11例原发性肿瘤和8例转移性肿瘤中进一步研究SPARC的表达。
结果
与对照组相比，用SPARC小干扰RNA转染的细胞系增殖显著降低。所有人类葡萄膜黑色素瘤病例以及异种移植模型的所有原发性和转移性肿瘤对SPARC均呈阳性染色。
结论
SPARC可能是抑制UM生长的一个新靶点。
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