用于胶束溶液中核磁共振研究的CB2的表达与纯化。
Expression and purification of CB2 for NMR studies in micellar solution.
作者信息
Krepkiy Dmitriy, Gawrisch Klaus, Yeliseev Alexei
机构信息
National Institute on Alcoholism and Alcohol Abuse, National Institutes of Health, Bethesda, MD 20892-9410, USA.
出版信息
Protein Pept Lett. 2007;14(10):1031-7. doi: 10.2174/092986607782541051.
We demonstrate feasibility of biophysical characterization of the peripheral cannabinoid receptor CB2 produced by heterologous expression in E. coli membranes. Recombinant receptor was purified by affinity chromatography, and NMR diffusion experiments performed on CB2 solubilized in dodecylphosphocholine (DPC) micelles. Circular dichroism spectroscopy indicated high alpha-helical content (49 %) of CB2.
我们证明了通过在大肠杆菌膜中进行异源表达来对外周大麻素受体CB2进行生物物理表征的可行性。重组受体通过亲和色谱法纯化，并对溶解在十二烷基磷酸胆碱（DPC）胶束中的CB2进行了核磁共振扩散实验。圆二色光谱表明CB2具有较高的α-螺旋含量（49%）。
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