S-腺苷甲硫氨酸在体外及对大鼠血管平滑肌细胞均能抑制泛素-蛋白酶体系统。

S-adenosylmethionine inhibits ubiquitin-proteasome system in vitro and on rat vascular smooth muscle cells.

作者信息

D'Anselmi Fabrizio, Cucina Alessandra, Cavallaro Giuseppe, Bizzarri Mariano, Cavallaro Rosaria Adele, Fuso Andrea, Cavallaro Antonino, Scarpa Sigfrido

机构信息

Department of Surgery P.Valdoni, University of Rome La Sapienza, Italy.

出版信息

Protein Pept Lett. 2008;15(1):58-62. doi: 10.2174/092986608783330396.

DOI:10.2174/092986608783330396

PMID:18221015

Abstract

S-adenosylmethionine is a metabolite regulating many biological processes; S-adenosylmethionine effect on ubiquitin-proteasome system (UPS) has not been studied yet. We investigated S-adenosylmethionine effects on UPS activity both in vitro, by inhibitor screening assay, and in rat vascular smooth muscle cells, by Western Blot of proteasomal targets. We found that S-adenosylmethionine inhibited UPS activity.

摘要

S-腺苷甲硫氨酸是一种调节多种生物过程的代谢物；尚未研究S-腺苷甲硫氨酸对泛素-蛋白酶体系统（UPS）的影响。我们通过抑制剂筛选试验在体外以及通过蛋白酶体靶标的蛋白质印迹法在大鼠血管平滑肌细胞中研究了S-腺苷甲硫氨酸对UPS活性的影响。我们发现S-腺苷甲硫氨酸抑制了UPS活性。