藤黄节杆菌限制性内切核酸酶识别序列及其对SV40 DNA的切割图谱。
Arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of SV40 DNA.
作者信息
Jay E, Wu R
出版信息
Biochemistry. 1976 Aug 10;15(16):3612-20. doi: 10.1021/bi00661a032.
PMID:182213
Abstract
The nucleotide sequence at the cleavage site of the restriction endonuclease isolated from Arthrobacter luteus (Alu) has been determined. The endonuclease cleaves at the center of a palindromic tetranucleotide sequence to give even-ended duplex DNA fragments phosphorylated at the 5'-end. The endonuclease cleaves SV40 form I DNA into 32 fragments. The order and sizes of these fragments have been determined to provide an Alu cleavage map of the SV40 genome.
摘要
已确定从黄色节杆菌（Alu）分离出的限制性内切核酸酶切割位点的核苷酸序列。该内切核酸酶在回文四核苷酸序列的中心进行切割，产生在5'端磷酸化的平端双链DNA片段。该内切核酸酶将SV40 I型DNA切割成32个片段。已确定这些片段的顺序和大小，以提供SV40基因组的Alu切割图谱。
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