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流感嗜血杆菌限制性内切酶对猴病毒40 DNA的特异性切割。

Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.

作者信息

Danna K, Nathans D

出版信息

Proc Natl Acad Sci U S A. 1971 Dec;68(12):2913-7. doi: 10.1073/pnas.68.12.2913.

DOI:10.1073/pnas.68.12.2913
PMID:4332003
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389558/
Abstract

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.

摘要

一种细菌限制性内切核酸酶已被用于产生SV40 DNA的特定片段。用流感嗜血杆菌的限制性内切核酸酶消化来自SV40噬菌斑纯化菌株的DNA，得到了11个可通过聚丙烯酰胺凝胶电泳分离的片段，其中8个与原始DNA等摩尔。通过电子显微镜、DNA含量或电泳迁移率测定，这些片段的分子量范围约为6.5×10⁵至7.4×10⁴道尔顿。

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