Jursinic P, Warden J
Biochim Biophys Acta. 1976 Aug 13;440(2):322-30. doi: 10.1016/0005-2728(76)90066-9.
In order to determine the major site of bicarbonate action in the electron transport complex of Photosystem II, the following experimental techniques were used: electron spin resonance measurements of Signal IIvf, measurements of chlorophyll a fluorescence yield rise and decay kinetics, and delayed light emission decay. From data obtained using these experimental techniques the following conclusions were made: (1) absence of bicarbonate causes a reversible inactivation of up to 40% of Photosystem II reaction center activity; (2) there is no significant effect of bicarbonate on electron flow from the charge accumulating S state to Z; (3) there is no significant effect of bicarbonate on electron flow from Z to P-680+; (4) electron flow from Q-- to the intersystem electron transport pool is inhibited by from 4- to 6-fold under bicarbonate depletion conditions.
为了确定碳酸氢盐在光系统II电子传递复合体中的主要作用位点，采用了以下实验技术：对信号IIvf进行电子自旋共振测量、测量叶绿素a荧光产量上升和衰减动力学以及延迟发光衰减。根据使用这些实验技术获得的数据得出了以下结论：（1）缺乏碳酸氢盐会导致高达40%的光系统II反应中心活性发生可逆失活；（2）碳酸氢盐对从电荷积累S态到Z的电子流动没有显著影响；（3）碳酸氢盐对从Z到P - 680+的电子流动没有显著影响；（4）在碳酸氢盐耗尽的条件下，从Q-到系统间电子传递池的电子流动受到4至6倍的抑制。
