Senthilkumar Tma, Subathra M, Phil M, Ramadass P, Ramaswamy V
Department of Animal Biotechnology, Madras Veterinary College, Chennai - 600 007, Tamil Nadu, India.
Indian J Med Microbiol. 2008 Jan-Mar;26(1):45-9. doi: 10.4103/0255-0857.38857.
Diagnosis of leptospirosis facilitates patient management and initiation of therapy. The microscopic agglutination test (MAT) is the serological test used in reference laboratories because of its high degree of sensitivity and specificity. But the results are not available quickly for patient management. In the present study, in order to develop a simple, rapid immunodiagnostic assay, one of the outer membrane proteins (OMPs), recombinant LipL41 (rLipL41) has been utilised in latex agglutination test (LAT) and flow-through assay.
Part of LipL41 gene was expressed in Escherichia coli system and purified. The rLipL41 antigen of pathogenic Leptospira interrogans serovar Icterohaemorrhagiae, which is conserved in all pathogenic Leptospira spp. was used as capture antigen in the LAT and flow-through test. Both tests are very rapid and could be completed within 5 minutes. The sensitivity and specificity of rLipL41 was assessed and evaluated in LAT and flow-through assay in comparison with standard MAT.
The sensitivity and specificity of the LAT were 89.70 and 90.45% and flow-through assay were 89.09 and 77.70%, respectively.
The developed LAT and flow-through assays were simple, rapid and economical for the detection of leptospira infection and suitable for large-scale screening of samples in endemic areas without any sophisticated equipment.
钩端螺旋体病的诊断有助于患者管理和治疗的启动。显微镜凝集试验（MAT）因其高度的敏感性和特异性，是参考实验室使用的血清学检测方法。但检测结果不能很快获得以供患者管理使用。在本研究中，为了开发一种简单、快速的免疫诊断方法，一种外膜蛋白（OMPs），重组LipL41（rLipL41）已被用于乳胶凝集试验（LAT）和流通试验。
LipL41基因的一部分在大肠杆菌系统中表达并纯化。致病性问号钩端螺旋体血清型出血热的rLipL41抗原在所有致病性钩端螺旋体属中保守，在LAT和流通试验中用作捕获抗原。这两种试验都非常快速，可在5分钟内完成。与标准MAT相比，在LAT和流通试验中评估和评价了rLipL41的敏感性和特异性。
LAT的敏感性和特异性分别为89.70%和90.45%，流通试验的敏感性和特异性分别为89.09%和77.70%。
所开发的LAT和流通试验用于检测钩端螺旋体感染简单、快速且经济，适用于在没有任何复杂设备 的流行地区对样本进行大规模筛查。
