Abu-Farha Mohamed, Elisma Fred, Figeys Daniel
Ottawa Institute of Systems Biology (OISB), University of Ottawa, 451 Smyth Road. Ottawa, K1H 8M5, Ontario, Canada.
Adv Biochem Eng Biotechnol. 2008;110:67-80. doi: 10.1007/10_2007_091.
The use of mass spectrometry in protein identification has revolutionized the field of proteomics. Coupled to various affinity purification techniques, mass spectrometry is used to identify protein-protein interactions. This chapter looks at the use of these affinity purification techniques in the identification of protein interactions. Various tags are used to purify protein complexes including tandem affinity purification. The FLAG tag is another commonly used tag which is a small tag that tends not to interfere with the protein function. These different affinity purification methods are used to purify proteins that are further identified by either ESI-MS or MALDI-MS.
质谱技术在蛋白质鉴定中的应用彻底改变了蛋白质组学领域。与各种亲和纯化技术相结合，质谱用于鉴定蛋白质 - 蛋白质相互作用。本章探讨了这些亲和纯化技术在蛋白质相互作用鉴定中的应用。各种标签用于纯化蛋白质复合物，包括串联亲和纯化。FLAG标签是另一种常用的标签，它是一种小标签，往往不会干扰蛋白质功能。这些不同的亲和纯化方法用于纯化蛋白质，然后通过电喷雾电离质谱（ESI-MS）或基质辅助激光解吸电离质谱（MALDI-MS）进一步鉴定。
