Kleinman H K
National Institute of Dental Research/NIH, Bethesda, Maryland, USA.
Curr Protoc Cell Biol. 2001 May;Chapter 10:Unit 10.2. doi: 10.1002/0471143030.cb1002s00.
This unit describes methods for passaging and harvesting the basement membrane matrix-producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiation of a variety of epithelial, endothelial, and neuronal cells. Procedures for the isolation of the adhesive glycoprotein laminin-1 and of type IV collagen are also included. Support protocols cover the maintenance and harvesting of EHS tumors in mice and maintenance of mice on a lathrogenic diet.
本单元介绍了传代培养和收获产生基底膜基质的EHS肿瘤的方法，以及随后分离称为基质胶的基底膜成分粗混合物的方法，基质胶可促进多种上皮细胞、内皮细胞和神经元细胞的分化。还包括分离黏附糖蛋白层粘连蛋白-1和IV型胶原蛋白的步骤。辅助方案涵盖了在小鼠中维持和收获EHS肿瘤以及在致跛性饮食条件下饲养小鼠。
