基底膜成分的相互作用。
Interactions of basement membrane components.
作者信息
Woodley D T, Rao C N, Hassell J R, Liotta L A, Martin G R, Kleinman H K
出版信息
Biochim Biophys Acta. 1983 Dec 27;761(3):278-83. doi: 10.1016/0304-4165(83)90077-6.
The binding of laminin, type IV collagen, and heparan sulfate proteoglycan to each other was assessed. Laminin binds preferentially to native type IV (basement membrane) collagen over other collagens. A fragment of laminin (Mr 600 000) containing the three short chains (Mr 200 000) but lacking the long chain (Mr 400 000) showed the same affinity for type IV collagen as the intact protein. The heparan sulfate proteoglycan binds well to laminin and to type IV collagen. These studies show that laminin, type IV collagen and heparan sulfate proteoglycan interact with each other. Such interactions in situ may determine the structure of basement membranes.
评估了层粘连蛋白、IV型胶原蛋白和硫酸乙酰肝素蛋白聚糖之间的结合情况。层粘连蛋白与天然IV型（基底膜）胶原蛋白的结合优先于其他胶原蛋白。含有三条短链（分子量200 000）但缺乏长链（分子量400 000）的层粘连蛋白片段（分子量600 000）对IV型胶原蛋白的亲和力与完整蛋白相同。硫酸乙酰肝素蛋白聚糖与层粘连蛋白和IV型胶原蛋白结合良好。这些研究表明层粘连蛋白、IV型胶原蛋白和硫酸乙酰肝素蛋白聚糖相互作用。这种原位相互作用可能决定基底膜的结构。
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