Akiyama S K
Chapel Hill, North Carolina, USA.
Curr Protoc Cell Biol. 2001 May;Chapter 10:Unit 10.6. doi: 10.1002/0471143030.cb1006s02.
This unit describes the purification of extracellular vitronectin from plasma by a series of affinity chromatography steps. First plasma is depleted of fibronectin and other heparin-binding proteins; the sample is then treated with urea to activate the heparin-binding activity of vitronectin. The resulting vitronectin is approximately 98% pure.
本单元介绍了通过一系列亲和色谱步骤从血浆中纯化细胞外玻连蛋白的方法。首先去除血浆中的纤连蛋白和其他肝素结合蛋白；然后用尿素处理样品以激活玻连蛋白的肝素结合活性。最终得到的玻连蛋白纯度约为98%。
