Akiyama Steven K
National Institute of Environmental Health Sciences, NIH, Research Triangle Park, North Carolina.
Curr Protoc Cell Biol. 2013 Sep 24;60:10.6.1-10.6.7. doi: 10.1002/0471143030.cb1006s60.
This unit describes the purification of extracellular vitronectin from plasma or serum by using heparin-affinity chromatography. First, the plasma is depleted of fibronectin plus other heparin- and Sepharose-binding proteins and treated with urea to activate the heparin-binding activity of vitronectin, which is subsequently bound to a heparin affinity column and eluted. The resulting vitronectin should be ∼ 98% pure.
本单元描述了通过肝素亲和色谱法从血浆或血清中纯化细胞外玻连蛋白的方法。首先，去除血浆中的纤连蛋白以及其他与肝素和琼脂糖结合的蛋白质，并用尿素处理以激活玻连蛋白的肝素结合活性，随后将其结合到肝素亲和柱上并洗脱。所得的玻连蛋白纯度应约为98%。
