间期非洲爪蟾卵提取物的制备与使用。
Preparation and use of interphase Xenopus egg extracts.
作者信息
Powers M, Evans E K, Yang J, Kornbluth S
机构信息
Emory University School of Medicine, Atlanta, Georgia, USA.
出版信息
Curr Protoc Cell Biol. 2001 May;Chapter 11:Unit 11.10. doi: 10.1002/0471143030.cb1110s09.
PMID:18228302
Abstract
In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.
摘要
在本实验中，从经激素处理的雌性非洲爪蟾中分离出卵，然后用环己酰亚胺处理提取物，使其停留在细胞周期的间期。在精子染色质和ATP存在的情况下，提取物中的膜泡融合形成细胞核，使提取物适用于DNA复制和核运输的研究。
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