Rhoads M L, Zarlenga D S, al-Yaman F M
United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705.
Southeast Asian J Trop Med Public Health. 1991 Dec;22 Suppl:268-70.
The 70% ammonium sulfate-soluble fraction of the cyst fluid of Taenia hydatigena (designated ThFAS) was previously shown to have potential as an immunodiagnostic reagent for bovine cysticercosis. Western blot analysis indicated that the specific reactivity with antibodies in sera of T. saginata-infected cattle was associated with a 10 kDa component. Rabbit antiserum to ThFAS identified a homologous antigenic protein from the cestode Taenia crassiceps. Consequently, a cDNA expression library was constructed in lambda gt11 using poly A mRNA purified from T. crassiceps metacestodes and screened with rabbit antiserum to ThFAS. One strongly reactive clone (designated lambda TCA-2) produced a 123 kDa beta-galactosidase fusion protein which reacted in Western blot with sera from calves experimentally-infected with T. saginata and did not react with sera from uninfected calves or from cattle infected with Fasciola hepatica or with common gastrointestinal cattle parasites.
泡状带绦虫囊液中70%硫酸铵可溶性部分（命名为ThFAS）先前已被证明有潜力作为牛囊尾蚴病的免疫诊断试剂。蛋白质印迹分析表明，与感染牛带绦虫的牛血清中的抗体的特异性反应与一个10 kDa的成分有关。针对ThFAS的兔抗血清鉴定出一种来自绦虫肥胖带绦虫的同源抗原蛋白。因此，使用从肥胖带绦虫中绦期幼虫纯化的聚腺苷酸mRNA构建了λgt11 cDNA表达文库，并用针对ThFAS的兔抗血清进行筛选。一个强反应性克隆（命名为λTCA-2）产生了一个123 kDa的β-半乳糖苷酶融合蛋白，该蛋白在蛋白质印迹中与经实验感染牛带绦虫的小牛血清发生反应，而与未感染小牛的血清、感染肝片吸虫的牛血清或常见的牛胃肠道寄生虫血清不发生反应。
