Hayunga E G, Sumner M P
Food Safety and Inspection Service, U.S. Department of Agriculture, Beltsville, MD 20705.
Vet Immunol Immunopathol. 1991 Mar;28(1):57-65. doi: 10.1016/0165-2427(91)90043-c.
An ammonium sulfate fraction of Taenia hydatigena cyst fluid (ThFAS) was further fractionated by hydrophobic interaction chromatography, using alkylagarose and omega-amino alkylagarose columns, in an effort to isolate and purify a specific diagnostic antigen in the ThFAS preparation. The less than 12 kDa antigen was found to have an affinity for immobilized alkanes with chain length of six carbons or greater. The antigen was recovered in an ethylene glycol eluate from a hexylagarose column then analyzed by Western blot; it reacted with bovine and human cysticercosis infection sera and with specific monoclonal antibodies but not with control sera or fascioliasis infection sera. When the eluate was used as coating antigen in a plate ELISA assay no false positive reactions were seen in sera from cattle infected with Fasciola hepatica; false positive reactions were observed for the unfractionated ThFAS antigen preparation.
为了从细粒棘球绦虫囊液硫酸铵级分（ThFAS）中分离和纯化一种特定的诊断抗原，使用烷基琼脂糖柱和ω-氨基烷基琼脂糖柱，通过疏水相互作用色谱对其进行了进一步分级分离。发现分子量小于12 kDa的抗原对链长为六个或更多碳原子的固定化烷烃具有亲和力。该抗原从己基琼脂糖柱的乙二醇洗脱液中回收，然后通过蛋白质免疫印迹法进行分析；它与牛和人的囊尾蚴病感染血清以及特异性单克隆抗体发生反应，但与对照血清或肝片吸虫病感染血清不发生反应。当将洗脱液用作平板ELISA试验中的包被抗原时，在感染肝片吸虫的牛血清中未观察到假阳性反应；而未分级的ThFAS抗原制剂则观察到假阳性反应。
