Sossountzov L, Ruiz-Avila L, Vignols F, Jolliot A, Arondel V, Tchang F, Grosbois M, Guerbette F, Miginiac E, Delseny M
Physiologie du Développement des Plantes, Centre National de la Recherche Scientifique (CNRS) (URA 1180), Université Pierre et Marie Curie, Paris, France.
Plant Cell. 1991 Sep;3(9):923-33. doi: 10.1105/tpc.3.9.923.
We studied the temporal and spatial pattern of lipid transfer protein (LTP) gene expression, as well as the localization of this protein, in maize. Using an LTP gene, we observed an accumulation of LTP mRNA in embryos and endosperms during seed maturation. LTP gene expression was also investigated in young seedlings. After germination, the level of LTP mRNA in the coleoptile increased, with a maximum at 7 days, whereas LTP mRNA levels were low in the scutellum and negligible in roots. The high levels of LTP mRNA found in coleoptiles and embryos were confirmed by in situ hybridization. Moreover, LTP gene expression appeared to be localized in the external cellular layers and around the leaf veins. Using immunogold methods, we also observed that LTP was distributed heterogeneously in the different cells of coleoptiles and leaves. The highest concentrations of LTP were found in the outer epidermis of the coleoptiles as well as the leaf veins. Together, our observations indicate that LTP gene expression is not only organ specific and time specific but also cell specific.
我们研究了玉米中脂类转移蛋白（LTP）基因表达的时空模式以及该蛋白的定位。利用一个LTP基因，我们观察到种子成熟过程中LTP mRNA在胚和胚乳中积累。我们还研究了幼苗中LTP基因的表达。萌发后，胚芽鞘中LTP mRNA的水平升高，在第7天达到最高，而盾片中LTP mRNA水平较低，根中则可忽略不计。原位杂交证实了胚芽鞘和胚中存在高水平的LTP mRNA。此外，LTP基因表达似乎定位于外部细胞层和叶脉周围。使用免疫金方法，我们还观察到LTP在胚芽鞘和叶片的不同细胞中分布不均一。在胚芽鞘的外表皮以及叶脉中发现LTP的浓度最高。总之，我们的观察结果表明，LTP基因表达不仅具有器官特异性和时间特异性，还具有细胞特异性。
