Kader J C, Julienne M, Vergnolle C
Eur J Biochem. 1984 Mar 1;139(2):411-6. doi: 10.1111/j.1432-1033.1984.tb08020.x.
A phospholipid transfer protein has been purified 195-fold from an extract of spinach leaves. This protein is capable of transferring phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol and phosphatidylethanolamine from liposomes to mitochondria. In addition to this protein, a minor part of the total activity was associated with a less purified fraction. The pure protein has an isoelectric point of 9.0 +/- 0.2 determined by a chromatofocusing technique. Electrophoresis on sodium dodecyl sulfate/polyacrylamide gel showed that the protein is homogeneous and has an apparent molecular weight of 9000 +/- 1000, in agreement with the value (8832) calculated from the amino acid composition. This composition is characterized by a high amount of alanine and glycine and by the absence of phenylalanine, whereas arginine, glutamine, histidine and methionine are minor components. The spinach protein is also able to transfer phosphatidylcholine and phosphatidylglycerol from liposomes to intact chloroplasts. This observation reinforces the hypothesis that plastid phospholipids are partly imported from outside the organelle by a transfer process.
一种磷脂转移蛋白已从菠菜叶提取物中纯化了195倍。这种蛋白质能够将磷脂酰胆碱、磷脂酰肌醇、磷脂酰甘油和磷脂酰乙醇胺从脂质体转移到线粒体。除了这种蛋白质外，总活性的一小部分与纯化程度较低的部分有关。通过色谱聚焦技术测定，纯蛋白的等电点为9.0±0.2。在十二烷基硫酸钠/聚丙烯酰胺凝胶上进行电泳表明，该蛋白质是均一的，表观分子量为9000±1000，与根据氨基酸组成计算的值（8832）一致。这种组成的特点是含有大量的丙氨酸和甘氨酸，且不含苯丙氨酸，而精氨酸、谷氨酰胺、组氨酸和蛋氨酸是次要成分。菠菜蛋白还能够将磷脂酰胆碱和磷脂酰甘油从脂质体转移到完整的叶绿体中。这一观察结果强化了这样一种假设，即质体磷脂部分是通过转移过程从细胞器外部导入的。
