Zhang Bao, Chen Hong, Hua Liushuai, Zhang Chunlei, Kang Xiangtao, Wang Xinzhuang, Pan Chuanying, Lan Xianyong, Lei Chuzhao
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Biochem Genet. 2008 Jun;46(5-6):362-8. doi: 10.1007/s10528-008-9152-z.
Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six breeds were 0.041-0.167. Polymorphism of the ND5 gene was shown to be associated with growth in the Nanyang breed. Individuals with haplotype B had greater hucklebone width than those with haplotype A when 6 months old (P < 0.01), as well as better body height, body length, body weight, and average daily gain at 6 months (P < 0.05). Therefore, haplotype B of the ND5 gene was likely to have a positive effect on growth traits at 6 months in the Nanyang breed.
采用聚合酶链式反应-单链构象多态性（PCR-SSCP）和DNA测序方法，对6个中国牛品种的714个个体的线粒体DNA ND5基因多态性进行了检测。结果显示，在P2位点检测到两种单倍型，命名为A和B，存在3个单核苷酸多态性（T12900C、A12923T、C12924T）。6个品种中单倍型A的频率为0.041-0.167。研究表明，ND5基因的多态性与南阳牛品种的生长相关。6月龄时，单倍型B的个体比单倍型A的个体具有更宽的髋骨宽度（P<0.01），以及更好的体高、体长、体重和6月龄时的平均日增重（P<0.05）。因此，ND5基因的单倍型B可能对南阳牛6月龄时的生长性状具有积极影响。
