Pang Y H, Lei C Z, Zhang C L, Lan X Y, Shao S, Gao X M, Wang J Q, Chen H
College of Animal Science and Technology, Northwest A and F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
Genet Mol Res. 2011 Mar 1;10(1):359-67. doi: 10.4238/vol10-1gmr1041.
We analyzed 20 exons, with their intron-exon boundaries, of the bovine Flt-1 gene, using a strategy combining PCR amplification and single-strand conformational polymorphism analysis (PCR-SSCP), followed by nucleotide sequence analysis, in 675 cattle. We then looked for associations between polymorphisms and growth traits. Twelve novel SNPs (ss#184956516, ss#184956517, ss#184956518, ss#184956519, ss#251343993, ss#251343994, ss#251343995, ss#251343996, ss#251343997, ss#251343998, ss#251343999, and ss#251344000) were detected in the bovine Flt-1 gene in all three breeds. We observed no significant associations between these polymorphisms and birth weight, body weight and average daily gain during different growth periods (6, 12, 18, and 24 months old) (P > 0.05), or in body height, body length, heart girth, or height at the hip in Nanyang cattle breeds.
我们采用聚合酶链反应扩增和单链构象多态性分析（PCR - SSCP）相结合的策略，随后进行核苷酸序列分析，对675头牛的牛Flt - 1基因的20个外显子及其内含子 - 外显子边界进行了分析。然后我们寻找多态性与生长性状之间的关联。在所有三个品种的牛Flt - 1基因中检测到12个新的单核苷酸多态性（ss#184956516、ss#184956517、ss#184956518、ss#184956519、ss#251343993、ss#251343994、ss#251343995、ss#251343996、ss#251343997、ss#251343998、ss#251343999和ss#251344000）。我们未观察到这些多态性与出生体重、体重以及不同生长阶段（6、12、18和24月龄）的平均日增重之间存在显著关联（P > 0.05），在南阳牛品种的体高、体长、胸围或臀高方面也未观察到显著关联。
