用于恰加斯病血清学诊断的凝胶扩散酶联免疫吸附测定（DIG-ELISA）

Diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) for Chagas' disease serodiagnosis.

Requejo H I, Nakamura P M, Vaz A J, Pialarissi C S, Hoshino-Shimizu S, Matsumoto T K, Nakamura H

Seção de Imunologia, Instituto Adolfo Lutz, São Paulo, Brasil.

Braz J Med Biol Res. 1991;24(5):471-83.

Abstract

Diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) was standardized and evaluated for the diagnosis of Chagas' disease, in comparison with the conventional serological tests indirect immunofluorescence (IFI), passive hemagglutination (PHA) and complement fixation (CF). 2. A total of 236 serum samples positive and negative for the serodiagnosis of Chagas' disease were studied. The group included 50 serum samples serologically positive for leishmaniasis and 36 positive for malaria. 3. The best diagnostic performance of DIG-ELISA was observed when serum samples were diluted to 1:8 and a diameter of zero mm (no color) was taken as the cut-off. Under these conditions, the relative indices of sensitivity, specificity and agreement were 100%. High positive correlation coefficients were obtained between DIG-ELISA and IFI (r1 = 0.9010), PHA (r2 = 0.8943) and CF (r3 = 0.8269). 4. We conclude that DIG-ELISA provides an alternative technique for screening chagasic infections, as well as for seroepidemiological surveys mainly because it is simple, easy to carry out and does not require expensive equipment.

摘要

对凝胶扩散酶联免疫吸附测定（DIG-ELISA）进行了标准化和评估，以用于恰加斯病的诊断，并与传统血清学检测间接免疫荧光法（IFI）、被动血凝试验（PHA）和补体结合试验（CF）进行比较。2. 共研究了236份恰加斯病血清学诊断阳性和阴性的血清样本。该组包括50份利什曼病血清学阳性的血清样本和36份疟疾阳性的样本。3. 当血清样本稀释至1:8且以零毫米直径（无颜色）作为临界值时，观察到DIG-ELISA的最佳诊断性能。在这些条件下，敏感性、特异性和一致性的相对指数均为100%。DIG-ELISA与IFI（r1 = 0.9010）、PHA（r2 = 0.8943）和CF（r3 = 0.8269）之间获得了高正相关系数。4. 我们得出结论，DIG-ELISA为筛查恰加斯感染以及血清流行病学调查提供了一种替代技术，主要是因为它简单、易于实施且不需要昂贵的设备。