Leeder Abigail C, Turner Geoffrey
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Fungal Genet Biol. 2008 Jun;45(6):897-911. doi: 10.1016/j.fgb.2007.12.001. Epub 2007 Dec 8.
BemA, the orthologue of Saccharomyces cerevisiae Bem1p, was identified through genome sequence comparison. We have shown that it plays a similar role to Bem1p in yeast, acting as a cell growth protein. Deletion of the gene produced a moderately abnormal hyphal tip morphology, and had an extremely detrimental effect on conidiospore production, with development stalling after conidiophore vesicle production. It was also shown that BemA is required for vacuole fusion, similar to Bem1p. This role is dependent on the first SH3 domain of the protein, whose deletion has no detectable effect on cell growth. Localisation studies showed that BemA formed a clear cap at hyphal tips, analogous to the S. cerevisiae polarisome. The relationship between BemA and SepA, a spitzenkörper protein, was investigated. It was found that localisation of the proteins were interdependent, and a conditional double mutant was inviable.
通过基因组序列比较鉴定出了酿酒酵母Bem1p的直系同源物BemA。我们已经表明，它在酵母中发挥着与Bem1p类似的作用，作为一种细胞生长蛋白。该基因的缺失产生了中度异常的菌丝尖端形态，并且对分生孢子的产生具有极其有害的影响，在分生孢子囊泡产生后发育停滞。还表明，与Bem1p类似，液泡融合需要BemA。该作用依赖于该蛋白的第一个SH3结构域，其缺失对细胞生长没有可检测到的影响。定位研究表明，BemA在菌丝尖端形成了一个清晰的帽，类似于酿酒酵母的极化体。研究了BemA与一种孢子体蛋白SepA之间的关系。发现这些蛋白的定位是相互依赖的，并且一个条件性双突变体是不可存活的。
