Upadhyay Srijana, Shaw Brian D
Program for Biology of Filamentous Fungi, Department of Plant Pathology and Microbiology, Texas A&M University, 2132 TAMU, College Station, TX 77843, USA.
Fungal Genet Biol. 2006 Nov;43(11):739-51. doi: 10.1016/j.fgb.2006.05.002. Epub 2006 Jun 22.
Upon germination Aspergillus nidulans swoM1 exhibits abnormal development by extending a primary germ tube that quickly reverts to isotropic growth and results in an enlarged, swollen apex with pronounced wall thickenings. Apical lysis occurs in 38% of the germlings. A point mutation in the AN6037.3 gene encoding the only phosphoglucose isomerase in A. nidulans is responsible for the defect. Loss of polarity is bypassed when glucose is replaced with alternate carbon sources but in all cases the mutant is unable to conidiate due to a block in conidiophore development at vesicle formation. In conidiophores SwoM::GFP localizes to multiple punctate, foci within each actively growing cell type, and to multiple foci in mature dormant conidia. In hyphae SwoM::GFP localized to two rings spanning the center of mature septa. In hyphae localization is concentrated at actively growing hyphal tips.
在萌发时，构巢曲霉swoM1通过延伸一条初级芽管表现出异常发育，该芽管会迅速恢复为各向同性生长，并导致顶端增大、肿胀，伴有明显的壁增厚。38%的幼苗会发生顶端溶解。构巢曲霉中唯一编码磷酸葡萄糖异构酶的AN6037.3基因中的一个点突变是导致该缺陷的原因。当用替代碳源取代葡萄糖时，极性丧失可被绕过，但在所有情况下，由于分生孢子梗发育在泡囊形成阶段受阻，突变体无法产孢。在分生孢子梗中，SwoM::GFP定位于每种活跃生长细胞类型内的多个点状焦点，以及成熟休眠分生孢子中的多个焦点。在菌丝中，SwoM::GFP定位于跨越成熟隔膜中心的两个环。在菌丝中，定位集中在活跃生长的菌丝顶端。
