Ponsot Elodie, Lexell Jan, Kadi Fawzi
Department of Health Sciences, Orebro University, Orebro, Sweden.
Muscle Nerve. 2008 Apr;37(4):467-72. doi: 10.1002/mus.20964.
We have previously shown that the number of satellite cells is lower in old than young men and women. The aim of this study was to further explore the effects of aging on the regenerative potential of skeletal muscle in 16 young and 26 old men and women with comparable physical activity level (young, 25 +/- 4 years; old, 75 +/- 4 years). Mean and minimum telomere lengths were determined using Southern blot analyses on biopsies obtained from the tibialis anterior muscle. There were no significant age or gender effects on mean and minimal telomeric lengths, suggesting that the replicative potential in the remaining satellite cells in the tibialis anterior muscle is not impaired with increasing age and the existence of in vivo regulatory mechanisms allowing the maintenance of telomere length. These results imply that moderate physical activity regularly performed by old subjects is not associated with accelerated telomere loss.
我们之前已经表明，老年男性和女性的卫星细胞数量低于年轻男性和女性。本研究的目的是进一步探讨衰老对16名年轻和26名老年男性及女性骨骼肌再生潜力的影响，这些受试者的身体活动水平相当（年轻组，25±4岁；老年组，75±4岁）。使用Southern印迹分析法对取自胫前肌的活检组织进行分析，以确定平均和最小端粒长度。年龄和性别对平均和最小端粒长度均无显著影响，这表明随着年龄增长，胫前肌中剩余卫星细胞的复制潜力并未受损，且存在体内调节机制可维持端粒长度。这些结果意味着老年受试者定期进行适度体育活动与端粒加速缩短无关。
