Nemeskéry A, Németh A, Sétáló G, Vigh S, Halász B
Cell Tissue Res. 1976 Jul 26;170(2):263-73. doi: 10.1007/BF00224303.
Adenohypophysial primordia of rat embryos at 13 to 15 days gestation were cultured in Parker 199 synthetic medium for 2 to 11 days. At the end of the culture period their fine structure and the presence of immunoreactive trophic hormones using the peroxidase-labeled antibody technique were investigated. The degree of differentiation in the glands depends largely on the age of the embryos furnishing the explants. Cultured pituitaries explanted on the 13th day of gestation contain only ACTH-positive cells and about 15% of the cells are granular. The granules are 50-100 nm in diameter in some cells, while in other cells they range from 50 to 200 nm. In cultivated adenohypophysial primordia of embryos on the 15th day of intrauterine life ACTH, prolactin, LH and TSH cells are evident, but only the same two kinds of granular cells can be observed with the electron microscope. The extent of cytodifferentiation in the glands explanted on the 14th day of gestation is intermediate between the two other groups. The data suggest that the fetal rat pituitary has the capacity of self-differentiation but to a lesser extent than that of the in situ hypophysis.
将妊娠13至15天的大鼠胚胎腺垂体原基在帕克199合成培养基中培养2至11天。培养期结束时，利用过氧化物酶标记抗体技术研究其精细结构和免疫反应性促激素的存在情况。腺体的分化程度很大程度上取决于提供外植体的胚胎的年龄。妊娠第13天移植的培养垂体仅含有促肾上腺皮质激素阳性细胞，约15%的细胞有颗粒。一些细胞中的颗粒直径为50 - 100纳米，而在其他细胞中，颗粒直径为50至200纳米。在子宫内生活第15天的胚胎培养腺垂体原基中，促肾上腺皮质激素、催乳素、促黄体生成素和促甲状腺激素细胞明显可见，但用电子显微镜只能观察到同样的两种颗粒细胞。妊娠第14天移植的腺体中细胞分化程度介于其他两组之间。数据表明，胎鼠垂体具有自我分化能力，但程度低于原位垂体。
