在主要的非洲疟疾媒介——对拟除虫菊酯具有抗性的斯氏按蚊中，一种细胞色素P450（CYP6P9）的过表达。

Over expression of a cytochrome P450 (CYP6P9) in a major African malaria vector, Anopheles Funestus, resistant to pyrethroids.

作者信息

Amenya D A, Naguran R, Lo T-C M, Ranson H, Spillings B L, Wood O R, Brooke B D, Coetzee M, Koekemoer L L

机构信息

Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private bag X 4, Sandringham, 2131, South Africa.

出版信息

Insect Mol Biol. 2008 Feb;17(1):19-25. doi: 10.1111/j.1365-2583.2008.00776.x.

DOI:10.1111/j.1365-2583.2008.00776.x

PMID:18237281

Abstract

Anopheles funestus Giles is one of the major African malaria vectors. It has previously been implicated in a major outbreak of malaria in KwaZulu/Natal, South Africa, during the period 1996 to 2000. The re-emergence of this vector was associated with monooxygenase-based resistance to pyrethroid insecticides. We have identified a gene from the monooxygenase CYP6 family, CYP6P9, which is over expressed in a pyrethroid resistant strain originating from Mozambique. Quantitative Real-Time PCR shows that this gene is highly over expressed in the egg and adult stages of the resistant strain relative to the susceptible strain but the larval stages showed almost no difference in expression between strains. This gene is genetically linked to a major locus associated with pyrethroid resistance in this A. funestus population.

摘要

冈比亚按蚊吉氏亚种是非洲主要的疟疾传播媒介之一。此前，它曾在1996年至2000年期间引发南非夸祖鲁/纳塔尔的一次重大疟疾疫情。这种媒介的再次出现与基于单加氧酶的拟除虫菊酯类杀虫剂抗性有关。我们从单加氧酶CYP6家族中鉴定出一个基因，即CYP6P9，它在源自莫桑比克的一个拟除虫菊酯抗性品系中过度表达。定量实时PCR显示，相对于敏感品系，该基因在抗性品系的卵和成虫阶段高度过度表达，但在幼虫阶段，品系间的表达几乎没有差异。该基因在遗传上与这个冈比亚按蚊种群中与拟除虫菊酯抗性相关的一个主要基因座相连。