Liu Yang, Wang Xiaomin, Luo Xiaoping, Wang Rui, Zhai Bintao, Wang Penglong, Li Junyan, Yang Xiaoye
School of Life Sciences, Ningxia University, Yinchuan 750021, China.
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Animals (Basel). 2023 Mar 3;13(5):919. doi: 10.3390/ani13050919.
A major problem faced by the agricultural industry is the resistance of to anthelmintic drugs. For a better understanding of the response of to IVM and for the screening of drug-resistance-related genes, we used RNA sequencing and isobaric tags for relative and absolute quantification (iTRAQ) technology to detect the transcriptomic and proteomic changes in after ivermectin treatment. An integrated analysis of the two omics showed that the differentially expressed genes and proteins were significantly enriched in the pathways of amino acid degradation, the metabolism of xenobiotics by cytochrome P450, the biosynthesis of amino acids, and the tricarboxylic acid cycle. We found that the upregulated UDP-glycosyltransferases (), glutathione S-transferase (), cytochrome P450 (), and p-glycoprotein () genes play important roles in drug resistance in . Our work will help in the understanding of the transcriptome and proteome changes in after IVM and will facilitate the discovery of genes related to drug resistance. This information can be further applied to increase the understanding of the response of IVM in relation to .
农业产业面临的一个主要问题是[对象]对驱虫药产生抗药性。为了更好地了解[对象]对伊维菌素（IVM）的反应以及筛选与抗药性相关的基因，我们使用RNA测序和相对与绝对定量等压标签（iTRAQ）技术来检测伊维菌素处理后[对象]的转录组和蛋白质组变化。对这两个组学的综合分析表明，差异表达的基因和蛋白质在氨基酸降解途径、细胞色素P450对外源生物的代谢、氨基酸生物合成以及三羧酸循环中显著富集。我们发现上调的尿苷二磷酸糖基转移酶（[具体酶名]）、谷胱甘肽S -转移酶（[具体酶名]）、细胞色素P450（[具体酶名]）和P -糖蛋白（[具体蛋白名]）基因在[对象]的抗药性中起重要作用。我们的工作将有助于了解伊维菌素处理后[对象]的转录组和蛋白质组变化，并将促进与抗药性相关基因的发现。这些信息可进一步用于增进对伊维菌素与[对象]相关反应的理解。
