对患有/未患有多发性硬化症的兄弟姐妹的B细胞进行微阵列分析——转录因子TCF2的作用
Microarray analysis in B cells among siblings with/without MS - role for transcription factor TCF2.
作者信息
Avasarala Jagannadha R, Chittur Sridar V, George Ajish D, Tine John A
机构信息
Multiple Sclerosis Specialty Care, Kansas Neurological Consultants, PA, Wichita, KS 67218, USA.
出版信息
BMC Med Genomics. 2008 Jan 31;1:2. doi: 10.1186/1755-8794-1-2.
BACKGROUND
We investigated if global gene expression and transcription networks in B-lymphocytes of siblings with multiple sclerosis (MS) were different from healthy siblings.
RESULTS
Using virus-transformed immortalized B cells and human whole genome bioarrays with validation using RT-qPCR, we found that in siblings with MS, genes for CXCL10, serpin B1 and FUT4 were up regulated whereas CDC5L, TNFRSF19 and HLA-DR were down regulated, among others; transcription analysis showed two intersecting clusters of transcriptional factors - the larger, governed by the upregulated transcription factor 2 (TCF2) and the smaller network regulated by the downregulated CDC5L.
CONCLUSION
No study has linked TCF2 to MS and to better understand the role of TCF2 in MS, studies in larger cohorts are required.
背景
我们研究了患有多发性硬化症（MS）的兄弟姐妹的B淋巴细胞中的全局基因表达和转录网络是否与健康的兄弟姐妹不同。
结果
使用病毒转化的永生化B细胞和人类全基因组生物芯片，并通过RT-qPCR进行验证，我们发现，在患有MS的兄弟姐妹中，CXCL10、丝氨酸蛋白酶抑制剂B1和FUT4等基因上调，而细胞分裂周期蛋白5样蛋白（CDC5L）、肿瘤坏死因子受体超家族成员19（TNFRSF19）和人类白细胞抗原DR（HLA-DR）等基因下调；转录分析显示了两个相交的转录因子簇——较大的簇由上调的转录因子2（TCF2）控制，较小的网络由下调的CDC5L调节。
结论
尚无研究将TCF2与MS联系起来，为了更好地理解TCF2在MS中的作用，需要在更大的队列中进行研究。
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