Suzuki Ryo, Oda Yusuke, Namai Eisuke, Takizawa Tomoko, Negishi Yoichi, Utoguchi Naoki, Tachibana Katsuro, Maruyama Kazuo
Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, Sagamihara City, Japan.
Yakugaku Zasshi. 2008 Feb;128(2):187-92. doi: 10.1248/yakushi.128.187.
In gene therapy, it is important to develop an effective and safe gene delivery system. Especially, from the viewpoint of reducing side effects, gene delivery into a specific site is essential. We previously, developed liposomal bubbles (Bubble liposomes) containing perfluoropropane. Bubble liposomes were useful as ultrasound enhanced gene delivery tools in vitro and in vivo. In this review, we introduced the characteristics of Bubble liposomes as ultrasound imaging agents and ultrasound enhanced gene delivery tools. Bubble liposomes worked as ultrasound imaging agents in cardiosonography. In addition, their combination with ultrasound exposure was able to deliver plasmid DNA in the femoral artery. The gene expression was only observed at the site of ultrasound exposure. Moreover, the gene delivery by Bubble liposomes and ultrasound exposure was more efficient than that by conventional lipofection method using Lipofectamine 2000. Therefore, it was suggested that Bubble liposomes might be a new class of tools for site specific gene delivery.
在基因治疗中，开发一种有效且安全的基因递送系统至关重要。特别是，从减少副作用的角度来看，将基因递送至特定部位是必不可少的。我们之前开发了含有全氟丙烷的脂质体气泡（气泡脂质体）。气泡脂质体在体外和体内作为超声增强基因递送工具非常有用。在这篇综述中，我们介绍了气泡脂质体作为超声成像剂和超声增强基因递送工具的特性。气泡脂质体在心脏超声检查中可作为超声成像剂。此外，它们与超声照射相结合能够在股动脉中递送质粒DNA。基因表达仅在超声照射部位被观察到。而且，气泡脂质体与超声照射相结合的基因递送比使用Lipofectamine 2000的传统脂质转染方法更有效。因此，有人提出气泡脂质体可能是一类用于特定部位基因递送的新型工具。
