Suzuki Ryo, Maruyama Kazuo
Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Methods Mol Biol. 2010;605:473-86. doi: 10.1007/978-1-60327-360-2_33.
Gene delivery with a physical mechanism using ultrasound (US) and nano/microbubbles is expected as an ideal system in terms of delivering plasmid DNA noninvasively into a specific target site. We developed novel liposomal bubbles (Bubble liposomes (BLs)) containing the lipid nanobubbles of perfluoropropane which were utilized for contrast enhancement in ultrasonography. BLs were smaller in diameter than conventional microbubbles and induced cavitation upon exposure ultrasound. In addition, when coupled with US exposure, BLs could deliver plasmid DNA into various types of cells in vitro and in vivo. The transfection efficiency with BLs and US was higher than that with conventional lipofection method. Therefore, the combination of BLs and US might be an efficient and novel nonviral gene delivery system.
利用超声（US）和纳米/微泡的物理机制进行基因递送有望成为一种理想的系统，能够将质粒DNA无创地递送至特定靶点。我们开发了新型脂质体气泡（气泡脂质体（BLs）），其包含全氟丙烷脂质纳米气泡，用于超声检查中的造影增强。BLs的直径小于传统微泡，在超声照射下会引发空化。此外，与超声照射相结合时，BLs能够在体外和体内将质粒DNA递送至各种类型的细胞。BLs与超声联合使用时的转染效率高于传统脂质转染方法。因此，BLs与超声的组合可能是一种高效且新颖的非病毒基因递送系统。
